LOCUS       40924_33617        3297 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pNTT104 DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3297)
  AUTHORS   Nomura N, Nagase T.
  TITLE     The nucleotide sequence of the pNTT104 vector
  JOURNAL   Published Only in Database (1999)
REFERENCE   2  (bases 1 to 3297)
  AUTHORS   Nomura N, Nagase T.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-1999) Nobuo Nomura, Kazusa DNA Research Institute,
            Laboratory of Gene Structure 1; 1532-3 Yana, Kisarazu, Chiba 
            292-0812, Japan (E-mail:nomura@kazusa.or.jp, 
            URL:http://www.kazusa.or.jp, Tel:81-438-52-3932, Fax:81-438-52-3931)
REFERENCE   3  (bases 1 to 3297)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3297)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Published 
            Only in Database (1999)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (31-AUG-1999) Nobuo Nomura, Kazusa DNA Research Institute, 
            Laboratory of Gene Structure 1"; volume: " 1532-3 Yana, Kisarazu, 
            Chiba 292-0812, Japan (E-mail:nomura@kazusa.or.jp, 
            URL:http://www.kazusa.or.jp, Tel:81-438-52-3932, Fax"; pages: 
            "81-438-52-3931"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     pNTT104
            This sequence is derived from the pUC118 sequence mainly compiled by
            changing the polylinker sequence (sequence at the position between 
            231 and 291 of the pUC118) to the 194-bp sequence (sequence at the 
            position between 231 and 426 of the pNTT104) containing multiple 
            restriction enzyme recognition sites. In addition to that, both the 
            ScaI and NarI sites of the pUC118 were eliminated by nucleotide 
            exchanges at the positions 586 (C to T) and 1802 (G to A) of the 
            pNTT104 sequence, respectively.
FEATURES             Location/Qualifiers
     source          1..3297
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    107..128
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        143..173
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(426..442)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      655..1110
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        1392..1496
                     /label=AmpR promoter
     CDS             1497..2354
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      2528..3116
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac ggcatgcctg
      241 caggcgccga cgtcatgcat gggccctcta gatctagtac tagtgatatc gatcggtccg
      301 tacgtttttc agcacgcgtt aaaaaaacca tccccctgga agcttaaggc ctcgagttcg
      361 aattcgcggc cgcacgtgtc catggatccc gggtcgacgg ccgcggtggc cggtaccgag
      421 ctcatactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt
      481 aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc
      541 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggcgcttgat gcggtatttt
      601 ctccttacgc atctgtgcgg tatttcacac cgcatacgtc aaagcaacca tagtacgcgc
      661 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
      721 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
      781 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
      841 tacggcacct cgaccccaaa aaacttgatt tgggtgatgg ttcacgtagt gggccatcgc
      901 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct
      961 tgttccaaac tggaacaaca ctcaacccta tctcgggcta ttcttttgat ttataaggga
     1021 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
     1081 attttaacaa aatattaacg tttacaattt tatggtgcac tctcagtaca atctgctctg
     1141 atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg
     1201 cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt
     1261 gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc
     1321 tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc
     1381 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     1441 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
     1501 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
     1561 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
     1621 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
     1681 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
     1741 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
     1801 aatactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
     1861 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
     1921 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     1981 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     2041 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     2101 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     2161 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
     2221 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     2281 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     2341 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
     2401 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     2461 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     2521 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     2581 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     2641 ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc
     2701 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     2761 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     2821 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     2881 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     2941 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     3001 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     3061 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     3121 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     3181 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     3241 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaag
//
